http://www.mpsciences.com/2021/04/23/dna-purification-processes-for-different-applications/
DNA purification is a step in the sample preparation workflow that eliminates enzymes, salts and other contaminants from lysed samples and products of PCR prior to applications like cloning and sequencing. It also eliminates unwanted PCR artifacts like primer dimers and nucleotides not integrated. DNA purification in molecular biological research is an essential step that requires careful planning to achieve reliable, high-quality results.
There are many different methods to eliminating DNA. Traditional DNA isolation methods involve various steps like leukocyte isolation or red blood cell lysis in order to remove hemoproteins that hinder the PCR reaction, deproteinization, the treatment of RNAse. These include ethanol and isopropanol precipitation and finally DNA elimination. The majority of these procedures require specialized equipment such as an electrophoresis machine and a biosafety cabinet due to the hazardous intercalating dyes used in the electrophoresis gel.
Other DNA purification techniques use spin columns or 96-well filter plates to separate out contamination by adsorbing them to the surface of the plate or column. These methods can be lengthy, especially if you are working with many samples or if the columns have to be manually refilled.
Dipsticks reduce the number of sample processing steps from six to three. They bind nucleic acid using a waxy cellulose-based material and release them when water is present. This method is especially useful in low resource settings, such as remote locations and teaching labs. Its simplicity (30 s per sample) makes it suitable for molecular diagnostic tests, such as detection of disease and genotype screening.